A simple and isothermal ligase-based amplification approach based on a ligation-activated cleavage reaction. Issue 89 (24th October 2018)

• Record Type: Journal Article
• Authors: Ma, Fei; Liu, Huan; Li, Chen-chen; Zhang, Chun-yang
• Is Part Of: Chemical communications. Volume 54:Issue 89(2018)
• Page Start: 12638
• Page End: 12641
• Publication Date: 2018-10-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc07843b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We develop a simple and isothermal ligase-based cyclic amplification approach for the sensitive detection of polynucleotide kinase, DNA, proteins and ATP. Abstract : We develop a simple and isothermal ligase-based cyclic amplification approach on the basis of a ligation-triggered duplex-specific nuclease (DSN)-mediated cleavage reaction without the involvement of any thermal cycling and separation procedures. As a proof of concept, we demonstrate the application of this method for the sensitive detection of polynucleotide kinase. Moreover, this method can be extended to the accurate quantification of DNA, proteins and ATP.