Preservation of Blood Vessels with an Oxygen Generating Composite. Issue 21 (1st October 2018)

• Record Type: Journal Article
• Authors: Zhang, Huaifa; Dalisson, Benjamin; Tran, Simon; Barralet, Jake
• Is Part Of: Advanced healthcare materials. Volume 7:Issue 21(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-01
• Subjects: biomaterials -- blood vessel functionality -- blood vessel preservation -- oxygen delivery -- peroxides; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201701338
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Damage caused by oxygen deficiency (hypoxia) is one of the major factors limiting tissue and organ preservation time. Cooling tissues slows down metabolic rate of cells thereby prolonging tissue and organ survival sufficiently to allow transport and transplantation within a few hours. Although metabolism is slowed, cells and some enzymes continue to consume oxygen that can render cold stored tissues hypoxic. Here, an oxygen‐generating composite (OGC) with sustained oxygen release is reported for ex vivo blood vessel preservation. Aorta segments are cultured under hypothermia for 25 days in vascular preservation media. The presence of OGC increases cell viability from 9 ± 6% to 96 ± 3% and retains 65 ± 8% of original KCl stimulated contractile force after 25 days compared with 25 ± 4% in controls. Culture for 7 days in nitrogen demonstrates proof‐of‐concept for normothermic blood vessel preservation, OGC increases the cell viability from 45 ± 15% to 78 ± 2%, and KCl stimulates contractile force from 49 ± 7% to 95 ± 8%, respectively. Oxygen release materials then may have a role in augmenting current preservation techniques. Abstract : An oxygen generating composite (OGC) for tissue preservation is reported. The OGC is a biocompatible microparticulate biocomposite able to release oxygen. The OGC is able to maintain high cell viability and KCl stimulated contractile force of blood vessels preserved at 4 °C for 25 days.