A highly sensitive fluorescent probe for selective detection of cysteine/homocysteine from glutathione and its application in living cells and tissues. (17th October 2018)

• Record Type: Journal Article
• Authors: Zhou, Ziyan; Duan, Guofeng; Wang, Yingying; Yang, Shikui; Liu, Xuyan; Zhang, Liying; Sun, Runing; Xu, Yungen; Gu, Yueqing; Zha, Xiaoming
• Is Part Of: New journal of chemistry. Volume 42:Number 22(2018)
• Page Start: 18172
• Page End: 18181
• Publication Date: 2018-10-17
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj04183k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A fluorescent probeNIPY-NBD was designed for detecting Cys/Hcy against GSH and applied in cell/tumor tissue imaging. Abstract : Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play significant roles in maintaining biological homeostasis in organisms. Due to their particular significance in the physiology and pathology of humans, accurate determination and differentiation of Cys, Hcy, and GSH for understanding their roles in related diseases are in urgent demand. Herein, a new fluorescent probeNIPY-NBD was designed and synthesized for the detection of Cys/Hcy and GSH. The probe exhibits a variety of excellent properties including high sensitivity and low cytotoxicity as well as good cell membrane permeability. Furthermore, the probe showed a 100-fold ( λ ex/em = 310 nm/520 nm) and 210-fold ( λ ex/em = 340 nm/550 nm) fluorescence enhancement when reacted with Cys in buffer, and could swiftly respond to Cys and Hcy within 2.5 min.NIPY-NBD was further applied successfully in imaging in A549 cells and xenograft mouse tumor tissues. Taken together, NIPY-NBD may have great potential in detecting and distinguishing Cys/GSH in living animals and could be applied in tumor diagnosis.