Effect of citric acid on setting reaction and tissue response to β-TCP granular cement. (24th February 2017)

• Record Type: Journal Article
• Authors: Fukuda, Naoyuki; Tsuru, Kanji; Mori, Yoshihide; Ishikawa, Kunio
• Is Part Of: Biomedical materials. Volume 12:Number 1(2017:Feb.)
• Publication Date: 2017-02-24
• Subjects: granular cement -- β-tricalcium phosphate -- retarder -- citric acid -- dicalcium phosphate dihydrate; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/aa5aea
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: We recently reported that when an acidic calcium phosphate solution is mixed with β -tricalcium phosphate ( β -TCP) granules, the resulting dicalcium phosphate dihydrate (DCPD) crystals form bridges between the β -TCP granules, creating a set interconnected porous structure in approximately 1 min. Although this self-setting β -TCP granular cement ( β -TCPGC) is useful for clinical applications, the short setting time is a key drawback for handling. In this study, the setting time of β -TCPGC was adjusted with the addition of citric acid, which is a known inhibiter of DCPD crystal growth. As the concentration of citric acid in the acidic calcium phosphate solution increased, the amount of DCPD formation in the set β -TCPGC decreased, and the crystal morphology of DCPD became elongated. β -TCPGC prepared with various citric acid concentrations were used as grafting material in rat calvarial bone defects to evaluate bone regeneration in vivo . Four weeks after implantation, no inflammatory reaction and approximately 20% new bone formation were observed, regardless of the presence or absence of citric acid in the liquid phase of β -TCPGC. We concluded, therefore, that citric acid might be a useful retarder of β -TCPGC setting times.