Distribution of omega-6 and omega-3 polyunsaturated fatty acids in the whole rat body and 25 compartments. (September 2015)

• Record Type: Journal Article
• Authors: Salem, N.M.; Lin, Y.H.; Moriguchi, T.; Lim, S.Y.; Salem, N.; Hibbeln, J.R.
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 100(2015:Sep.)
• Page Start: 13
• Page End: 20
• Publication Date: 2015-09
• Subjects: ATL adrenal gland, thyroid and mandibular lymph nodes -- ADB brown adipose -- ADW white adipose -- BHT butylated hydroxytoluene -- DHA docosahexaenoic acid -- DPA docosapentaenoic acid -- EFA essential fatty acids -- EPA eicosapentaenoic acid -- FAME fatty acid methyl esters -- FID flame ionization detector -- GC gas–liquid chromatography -- HUFA highly unsaturated fatty acids -- MS mass spectrometry -- PUFA polyunsaturated fatty acids -- RBC red blood cell -- SG salivary glands -- SPC spinal cord -- TFA total fatty acid -- wt% weight percent; Alpha-linolenic acid -- Linoleic acid -- Docosahaexenoic acid -- Arachidonic acid -- n−3 -- n−6; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2015.06.002
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: The steady state compositions of omega-6 and omega-3 polyunsaturated fatty acids (PUFA) throughout the various viscera and tissues within the whole body of rats have not previously been described in a comprehensive manner. Dams consumed diets containing 10 wt% fat (15% linoleate and 3% α-linolenate). Male offspring ( n =9) at 7-week of age were euthanized and dissected into 25 compartments. Total lipid fatty acids for each compartment were quantified by GC/FID and summed for the rat whole body; total n −6 PUFA was 12 wt% and total n −3 PUFA was 2.1% of total fatty acids. 18:2 n −6 accounted for 84% of the total n −6 PUFA, 20:4 n −6 was 12%, 18:3 n −3 was 59% of the total n −3 PUFA, 20:5 n −3 was 2.1%, and 22:6 n −3 was 32%. The white adipose tissue contained the greatest amounts of 18:2 n −6 (1.5 g) and 18:3 n −3 (0.2 g). 20:4 n −6 was highest in muscle (60 mg) and liver (57 mg), while 22:6 n −3 was greatest in muscle (46 mg), followed by liver (27 mg) and carcass (20 mg). In terms of fatty acid composition expressed as a percentage, 18:2 n −6 was the highest in the heart (13 wt%), while 18:3 n −3 was about 1.3 wt% for skin, white adipose tissue and fur. 20:4 n −6 was highest (21–25 wt%) in the circulation, kidney, and spleen, while 22:6 n −3 was highest in the brain (12 wt%), followed by the heart (7.9 wt%), liver (5.9 wt%), and spinal cord (5.1 wt%). Selectivity was greatest when comparing 22:6 n −3 in brain (12%) to white adipose (0.08%) (68-fold) and 22:5 n −6 in testes (15.6%) compared to white adipose (0.02%), 780-fold.