(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity. (20th November 2015)

• Record Type: Journal Article
• Authors: Oliveira, Kassandra S.M.; Di Bastiani, Mirela; Cordeiro, Lucimara M.C.; Costa, Mírian F.; Toledo, Karina A.; Iacomini, Marcello; Babosa, Aneli M.; Dekker, Robert F.H.; Nascimento, Valéria M.G.
• Is Part Of: Carbohydrate polymers. Volume 133(2015)
• Page Start: 539
• Page End: 546
• Publication Date: 2015-11-20
• Subjects: Lasiodiplodan -- Linear (1→6)-β-glucan -- (1→3)(1→6)-β-Glucan -- Exopolysaccharides -- Immunomodulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.07.060
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8 × 10 6 Da and 7.0 × 10 3 Da, respectively. From a total of 2.2 g/L of EPS produced by L. theobromae through submerged fermentation, 1.5 g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.