High plasma concentrations of dolutegravir in patients with ABCG2 genetic variants. Issue 11 (November 2017)

• Record Type: Journal Article
• Authors: Tsuchiya, Kiyoto; Hayashida, Tsunefusa; Hamada, Akinobu; Oki, Sakurako; Oka, Shinichi; Gatanaga, Hiroyuki
• Is Part Of: Pharmaocogenetics and genomics. Volume 27:Issue 11(2017:Nov.)
• Publication Date: 2017-11
• Subjects: antiretroviral therapy -- ATP-binding cassette transporter B1 -- ATP-binding cassette transporter G2 -- dolutegravir -- plasma concentrations; Pharmacogenetics -- Periodicals; Pharmacogenomics -- Periodicals; Genetic toxicology -- Periodicals; Biomedical genetics -- Periodicals; 615.7
• DOI: 10.1097/FPC.0000000000000308
• Languages: English
• ISSNs: 1744-6872

Abstract

Abstract : The ATP-binding cassette transporters B1 (ABCB1) and G2 (ABCG2) are both expressed in the intestine and known as efflux transporters of drugs. Dolutegravir was identified recently as a substrate of both ABCB1 and ABCG2. This study aimed to determine the relations between single-nucleotide polymorphisms of ABCB1 and ABCG2 genes and plasma dolutegravir concentrations. Plasma samples were obtained from 42 HIV-1-infected patients treated with dolutegravir-containing regimens 0.5–4 h after dolutegravir dosing. Plasma dolutegravir concentrations were measured by liquid chromatography–mass spectrometry. Genomic DNA was isolated from peripheral blood mononuclear cells. Genotyping of allelic variants of ABCB1 1236 C>T (rs1128503), 2677 G>T/A (rs2032582), 3435 C>T (rs1045642), 4036 A>G (rs3842), and ABCG2 421 C>A (rs2231142) was performed using the TaqMan drug metabolism assays. None of the genotypes in ABCB1 1236 C>T, 2677 G>T/A, 3435 C>T, and 4036 A>G correlated with plasma dolutegravir concentration. In contrast, the mean peak plasma concentration of dolutegravir was significantly higher in the genotypes of ABCG2 421 AA (5002 ng/ml, n =3) compared with the genotypes of ABCG2 421 CC (2569 ng/ml, n =22) and ABCG2 421 CA (2479 ng/ml, n =17) ( P =0.0005). The speculated peak level of plasma dolutegravir concentration was significantly higher in ABCG2 genetic variant holders, probably, at least in part, because of low expression levels of efflux transporters in the intestines associated with these genetic variants.