Expression of p63 immunostaining in liquid‐based cytology (BD SurePath) of breast fine‐needle aspiration. Issue 10 (25th July 2018)

• Record Type: Journal Article
• Authors: Osugi, Masumi; Kinoshita, Kosei; Sugita, Atsuro; Kito, Katsumi; Maeda, Toshiharu
• Is Part Of: Diagnostic cytopathology. Volume 46:Issue 10(2018)
• Page Start: 845
• Page End: 852
• Publication Date: 2018-07-25
• Subjects: breast pitfall -- fine‐needle aspiration cytology -- liquid‐based cytology -- myoepithelial cell -- p63 immunocytochemistry; Cytodiagnosis -- Periodicals; Pathology, Cellular -- Periodicals; Cytology -- Periodicals; 616.07582
• DOI: 10.1002/dc.24041
• Languages: English
• ISSNs: 8755-1039

Abstract

Abstract : Background: We performed p63 immunostaining to detect myoepithelial cells on BD SurePath liquid‐based cytology (LBC) slides and examined whether this improved the diagnostic accuracy in breast fine‐needle aspiration cytology (FNAC). Methods: We examined the diagnostic accuracy using the LBC‐p63 immunostaining slides of 298 lesions obtained from July 2010 to August 2016. Results: We defined the cutoff values for malignancy as follows: (1) the percentage of p63+ cluster was <30%, (2) the percentage of p63+ single cells in cell clusters was <3%, (3) the number of p63+ single cells in the background was <20 when the total number of the cell clusters was <100, or the number was <120 when the total number was ≥100. The malignant lesions showed significantly lower values than the benign lesions in the percentage of p63+ clusters, the percentage of p63+ single cells in the clusters, and the number of p63+ single cells in the background ( P < .001). The diagnostic values obtained only by Papanicolaou staining vs the improved values with LBC‐p63 immunostaining slides were as follows. Sensitivity, 88.3% to 99.0%; specificity, 89.1% to 99.0%; positive predictive value, 94.8% to 99.5%; negative predictive value, 77.4% to 97.8%; diagnostic accuracy, 88.6% to 99.0%, respectively. Conclusion: The diagnostic accuracy of breast FNAC was significantly improved by adding LBC‐p63 immunostaining.