Theoretical aspects of peptide imprinting: screening of MIP (virtual) binding sites for their interactions with amino acids, di- and tripeptides. Issue 3 (3rd July 2018)

• Record Type: Journal Article
• Authors: Settipani, Julie; Karim, Kal; Chauvin, Alienor; Ibnou-Ali, Si Mohamed; Paille-Barrere, Florian; Mirkes, Evgeny; Gorban, Alexander; Larcombe, Lee; Whitcombe, Michael J.; Cowen, Todd; Piletsky, Sergey A.
• Is Part Of: Journal of the Chinese Advanced Materials Society. Volume 6:Issue 3(2018)
• Page Start: 301
• Page End: 310
• Publication Date: 2018-07-03
• Subjects: Molecularly imprinted polymers -- synthetic receptors -- computational design -- peptide imprinting -- molecular modelling; Materials -- Periodicals; Materials science -- Periodicals; Materials; Periodicals; 620.1105
• DOI: 10.1080/22243682.2018.1467279
• Languages: English
• ISSNs: 2224-3682

Abstract

ABSTRACT: Molecular modelling and computational approaches were used to design (virtual) molecularly imprinted binding sited for 170 amino acids, dipeptides and tripeptides. Analysis of the binding energy of ligands to their corresponding virtual binding sites revealed a direct correlation between size of the ligand and its binding affinity. Only tripeptides were capable of forming binding sites in molecularly imprinted polymers (MIPs) that are capable, in theory, of binding the corresponding targets at micromolar concentrations. No appreciable specificity was demonstrated in binding of virtual binding sites and corresponding templates. It is possible to conclude that although tripeptide sequences are sufficiently long to form MIPs with relatively high affinity, the sequence of peptide epitopes should be substantially longer that three amino acid residues to ensure specificity of imprinted sites. This consideration will be useful for the design of highly efficient MIPs for proteins. Abstract :