Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine−polyglycine for highly efficient gene delivery in vitro and in vivo. (5th October 2018)

• Record Type: Journal Article
• Authors: Guo, Zhaopei; Lin, Lin; Chen, Jie; Zhou, Xingzhi; Chan, Hon Fai; Chen, Xuesi; Tian, Huayu; Chen, Meiwan
• Is Part Of: Biomaterials science. Volume 6:Number 11(2018)
• Page Start: 3053
• Page End: 3062
• Publication Date: 2018-10-05
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c8bm00503f
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : The highly efficient gene delivery system with effective serum resistant capacity is promising for cancer therapy. Abstract : To improve gene transfection efficiency in vitro and in vivo, we first grafted polyglycine (PGly) onto branched polyethyleneimine (PEI) (molecular weight M W 25 kDa) to obtain a novel gene vector PGly−PEI (PPG), before complexing it with poly(ethylene glycol)-poly-l -glutamate (PEG-PLG) for adjusting the charge density of PEI. We named the resulting nano-polyplex PG/PPG. The PPGs demonstrated improved gene transfection efficiency after grafting with polyglycine, and the efficiency could be further increased with the introduction of PEG-PLG. The PG/PPG exhibited not only excellent DNA and siRNA transfection capacity in various cell lines including HeLa, CHO, HepG2, MCF7 and Huh7, but also reduced cytotoxicity compared with PEI. Besides, PG/PPG induced a higher degree of cell apoptosis when mixed genes of pKH3-rev-casp-3 plasmid and siBcl2 were used rather than either of them. Furthermore, PG/PPG was highly resistant to serum, which led to enhanced in vivo red fluorescent protein (RFP) expression in HeLa tumors. These results suggested that PG/PPG is a promising gene carrier for highly efficient gene delivery.