Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer. Issue 21 (28th September 2018)

• Record Type: Journal Article
• Authors: Shimada, Taisuke; Yasui, Takao; Yokoyama, Asami; Goda, Tatsuro; Hara, Mitsuo; Yanagida, Takeshi; Kaji, Noritada; Kanai, Masaki; Nagashima, Kazuki; Miyahara, Yuji; Kawai, Tomoji; Baba, Yoshinobu
• Is Part Of: Lab on a chip. Volume 18:Issue 21(2018)
• Page Start: 3225
• Page End: 3229
• Publication Date: 2018-09-28
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc00438b
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Biomolecular recognition can be attained by using the self-assembled monolayer modification for capture of a target analyte on the nanowire surfaces instead of primary antibodies. Abstract : Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.