Leveraging the IncuCyte Technology for Higher-Throughput and Automated Chemotaxis Assays for Target Validation and Compound Characterization. (February 2018)

• Record Type: Journal Article
• Authors: Chen, Jing; Ribeiro, Bert; Li, Han; Myer, Larnie; Chase, Peter; Surti, Neha; Lippy, Jonathan; Zhang, Litao; Cvijic, Mary Ellen
• Is Part Of: SLAS discovery. Volume 23:Number 2(2018)
• Page Start: 122
• Page End: 131
• Publication Date: 2018-02
• Subjects: higher throughput -- automated chemotaxis platform -- kinetic cell motility -- target validation -- compound characterization -- cell trafficking -- automation or robotics -- cell-based assays -- imaging technologies -- immunoassays; Drugs -- Analysis -- Periodicals; Drugs -- Testing -- Periodicals; Biomolecules -- Analysis -- Periodicals; Biomolecules -- Analysis; Drugs -- Analysis; Drugs -- Testing; Drug Evaluation, Preclinical; Molecular Biology -- methods; Periodicals; Periodicals; 615.1
• DOI: 10.1177/2472555217733437
• Languages: English
• ISSNs: 2472-5552

Abstract

Chemotaxis is the directional movement of cells in response to a chemical stimulus and is vital for many physiological processes, including immune responses, tumor metastasis, wound healing, and blood vessel formation. Therefore, modulation of chemotaxis is likely to be of therapeutic benefit. Hence, a high-throughput means to conduct chemotaxis assays is advantageous for lead evaluation and optimization in drug discovery. In this study, we have validated a novel approach for a higher-throughput, label-free, image-based IncuCyte chemotaxis assay encompassing various cell types, including T cells, B cells, mouse Th17, immature and mature dendritic cells, monocyte THP-1, CCRF-CEM, monocytes, neutrophils, macrophages, and MDA-MB-231. These assays enable us to visualize chemotactic cell migration in real time and perform kinetic cell motility studies on an automated platform, thereby allowing us to incorporate the quantitative studies of cell migration behavior into a routine drug discovery screening cascade.