Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer. Issue 86 (12th October 2018)

• Record Type: Journal Article
• Authors: Elfaramawy, Maie A.; Fujii, Satoshi; Uyeda, Atsuko; Osaki, Toshihisa; Takeuchi, Shoji; Kato, Yasuhiko; Watanabe, Hajime; Matsuura, Tomoaki
• Is Part Of: Chemical communications. Volume 54:Issue 86(2018)
• Page Start: 12226
• Page End: 12229
• Publication Date: 2018-10-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc06804f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Quantification of membrane proteins supplied by cell-free synthesis was achieved by using an easy-to-use droplet interface bilayer chamber model. Abstract : We report the functional synthesis and quantification of membrane proteins—α-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli— at the stabilized droplet interface bilayer using an in vitro transcription–translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.