Use of agricultural waste (coconut shell) for the synthesis of silver nanoparticles and evaluation of their antibacterial activity against selected human pathogens. (November 2018) - Sinsinwar, Simran
Abstract: Green synthesis of silver nanoparticles (AgNPs) is environmentally satisfactory because of their low cost and safe to nature. In the present study, extract of an agricultural waste, coconut ( Cocos nucifera ) shell is used to synthesize AgNPs and their antibacterial effect was investigated against selected human pathogens Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Salmonella typhimurium . The AgNPs synthesized using coconut shell extract (CSE-AgNPs) were characterized using UV–Visible spectroscopy (absorption peak at 432 nm), Transmission Electron Microscopy (spherical shaped particles size of 14.2–22.96 nm), Fourier-Transform Infrared Spectroscopy indicating the CSE capping around the AgNPs (Peaks 1384, 1609 and 3418 corresponds to organic molecules) and X-Ray Diffraction (Peak at 32.078 and 2-Theta). CSE-AgNPs exhibited zone of inhibition against S. aureus (15 mm), E. coli (13 mm), S. typhimurium (13 mm) and L. monocytogenes (10 mm) and minimum inhibitory concentration (MIC) of 26, 53, 106 and 212 μg/ml, respectively. Growth curve assay showed the effectiveness of CSE-AgNPs to inhibit the selected pathogens when compared to amphicillin control and extract. Scanning electron microscopy results indicated that the cell wall degradation might be the possible mechanism of antibacterial action of CSE-AgNPs. Different concentrations of AgNPs (0.078–2.5 mg/ml) showed no toxicity against human PBMC cell line. Hence, such highly effective CSE-AgNPs could be explored as antibacterial agent. Highlights: First time we have reported the use of agricultural waste (coconut shell) for the green synthesis of silver nanoparticles. Nanocharacteristics of AgNPs were confirmed by using UV-VIS spectroscopy, TEM, FTIR and XRD. Antibacterial activity and cell wall degradation mechanism of CSE-AgNPs were shown against selected human pathogens.