Body fat modulated activity of Gallus gallus domesticus Linnaeus (1758) and Meleagris gallopavo Linnaeus (1758) in association with antibiotics against bacteria of veterinary interest. (November 2018)

• Record Type: Journal Article
• Authors: de Queiroz Dias, Diógenes; Sales, Débora Lima; Andrade, Jacqueline Cosmo; Pereira da Silva, Ana Raquel; Tintino, Saulo Relison; Datiane de Morais Oliveira-Tintino, Cícera; de Araújo Delmondes, Gyllyandeson; Gadelha Rocha, Marcos Fábio; Martins da Costa, José Galberto; Romeu da Nóbrega Alves, Rômulo; Ferreira, Felipe Silva; Melo Coutinho, Henrique Douglas; de Oliveira Almeida, Waltécio
• Is Part Of: Microbial pathogenesis. Volume 124(2018)
• Page Start: 163
• Page End: 169
• Publication Date: 2018-11
• Subjects: Ethnoveterinary -- Zootherapy -- Fatty acids -- Antibiotic modulating activity; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.08.029
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: In the Northeast of Brazil, ethnoveterinary studies have shown that the body fat from Gallus gallus domesticus and Meleagris gallopavo are used for diseases that affect domestic animals. The objective of this study was to identify the chemical composition and to evaluate the antibacterial activity of the Gallus gallus domesticus (OFGG) and Meleagris gallopavo (OFMG) fixed oils in isolation and in association with antibiotics. The OFGG and OFMG from the poultry's body fat were extracted using hexane as a solvent in Soxhlet. Their composition was indirectly determined using fatty acid methyl esters. The OFGG and OFMG antibacterial and modulatory activities against standard and multi-resistant bacterial strains were performed through the broth microdilution test. In the OFGG chemical composition, 4 constituents were identified. The saturated fatty acid (AGS) and unsaturated fatty acid (AGI) percentages were 35.1% and 64.91% respectively, with linoleic acid being the major component. In the OFMG, 3 constituents were identified. The AGS percentage was 27.71% and 72.29% for AGI, with oleic acid as the most abundant component. The oils did not present antibacterial activity when tested in isolation, presenting Minimum Inhibitory Concentrations (MICs) > 512 μg/mL. However, when associated with antibiotics the OFGG showed synergistic activity with the antibiotics Amikacin, Amoxicillin, Norfloxacin and Oxytetracycline, while the OFMG promoted a synergistic action with the antibiotics Amikacin, Amoxicillin and Norfloxacin. Highlights: The OFGG and OFMG did not present clinically relevant antibacterial activity. The OFGG modulated synergistically the action of the antibiotics Amikacin, Amoxicillin, Norfloxacin, and Oxytetracycline; . The OFMG modulated synergistically the action of Amikacin, Amoxicillin, and Norfloxacin.