Extract from Moringa oleifera seeds suppresses the epithelial-mesenchymal transition-mediated metastasis of gastric cancer by targeting the metastatic suppressor NDRG1. (November 2018)

• Record Type: Journal Article
• Authors: Shu, Xing-sheng; Wang, Dan-dan; Zhao, Ying-ying; Sun, Yan-mei; Hu, Xiao-peng; Huang, Xiao-yan; Zeng, Jun-hui; Li, Chen-yang; Li, Yan; Zeng, Yong; Wang, Xiao-mei; He, Zhen-dan; Ying, Ying
• Is Part Of: Journal of functional foods. Volume 50(2018)
• Page Start: 93
• Page End: 103
• Publication Date: 2018-11
• Subjects: MO -- Gastric cancer -- Metastasis -- EMT -- NDRG1; CCK-8 cell counting kit-8 -- DEGs differentially expressed genes -- EdU 5-ethynyl-2′-deoxyuridine -- EMT epithelial-mesenchymal transition -- FDR false discovery rate -- GC gastric cancer -- GSEA gene set enrichment analysis -- HPLC High Performance Liquid Chromatography -- LC/MS Liquid Chromatography Mass Spectrometry -- LEF-1 lymphoid-enhancing factor-1 -- M. oleifera Moringa oleifera Lam -- MO residues of M. oleifera -- NDRG1 N-Myc Downstream Regulated 1 -- PI propidium iodide -- siRNA short Interfering RNA -- SEM standard error of the mean -- TCF T cell factor; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.09.030
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Moringa oleifera extracts (MO) suppresses metastasis of gastric cancer (GC) cells. MO reverses the epithelial-mesenchymal transition (EMT) in GC cells. A metastasis suppressor NDRG1 is upregulated in MO-treated GC cells. NDRG1 silencing blocks the inhibitory effect of MO on EMT-mediated metastasis. Abstract: Metastasis is the major feature of gastric cancer (GC) that contributes to poor prognosis. There is an urgent need to identify novel agents targeting GC metastasis. Moringa oleifera Lam ( M. oleifera ) has long been used by the public as foods for its nutritional and medicinal virtues. However, its anti-metastatic potential against GC remains unknown. Here, we showed that residues of M. oleifera (MO) can specifically suppress metastasis of GC cells at a lower dosage, at least partially through reversing the epithelial-mesenchymal transition (EMT). Furthermore, RNA-sequencing analysis revealed that a bona fide metastasis suppressor, NDRG1, was significantly upregulated by MO treatment. Knockdown of NDRG1 by a short interfering RNA dramatically attenuated the anti-metastasis effect of MO in GC cells. Thus, we demonstrate that MO suppresses EMT-mediated metastasis of GC by augmenting the metastatic suppressor NDRG1. Our findings also address the potential therapeutic significance of MO in the treatment of GC metastasis.