The antibacterial and physiological effects of pure and nanoencapsulated Origanum majorana essential oil on fish infected with Aeromonas hydrophila. (November 2018)

• Record Type: Journal Article
• Authors: da Cunha, Jessyka Arruda; de Ávila Scheeren, Cecília; Fausto, Viviane Pedroso; de Melo, Larissa Daiane Willrich; Henneman, Bruno; Frizzo, Clarissa Piccinin; de Almeida Vaucher, Rodrigo; Castagna de Vargas, Agueda; Baldisserotto, Bernardo
• Is Part Of: Microbial pathogenesis. Volume 124(2018)
• Page Start: 116
• Page End: 121
• Publication Date: 2018-11
• Subjects: Marjoram -- Nanotechnology -- Rhamdia quelen -- Silver catfish; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.08.040
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: This study evaluated the antibacterial activity of Origanum majorana essential oil (EOM) and nanocapsules of this oil (NOM) in silver catfish, Rhamdia quelen, infected with Aeromonas hydrophila, and addressed their effects on silver catfish hematological and metabolic parameters. Fish were inoculated with A. hydrophila (360 μL, at a concentration of 1.5 × 10 9 CFU mL −1 ) and submitted to 1 h daily baths with EOM (0 (control), 20 or 30 μL L −1 ), NOM (0 (control), 5 or 10 μL L −1 ) or a positive control containing florfenicol (30 μL L −1 ) called group Maxflor ® for five consecutive days. All treatments improved the survival rate of the infected fish, but we suggest the treatment of A. hydrophila infections through daily baths with 20 μL L −1 EOM or 5 μL L −1 NOM for five consecutive days as these were the lowest effective concentrations tested. Silver catfish treated with EOM and NOM had higher lymphocyte levels, indicating stimulation of the immune system in these fish. The lowest liver glucose level was found in the group treated with the lowest concentration of NOM, and the lactate values in the liver and muscle of all groups were within the normal values reported for this species. In addition, nanocapsules required much less EOM to elicit effective antibacterial treatment. Highlights: Origanum majorana essential oil may minimize and/or inhibit bacterial effect. The effect of pure and nanocapsule forms was observed by bathing infected fish. Both forms demonstrated an antibacterial effect against Aeromonas hydrophila. Nanocapsules of the essential oil presented better efficacy than the pure form.