IQGAP1 silencing suppresses the malignant characteristics of laryngeal squamous cell carcinoma cells. Issue 1 (January 2018)

• Record Type: Journal Article
• Authors: Wang, Xiaoxia; He, Chun; Li, Chaohui; Ren, Benhong; Deng, Qing; Gao, Wei; Wang, Binquan
• Is Part Of: International journal of biological markers. Volume 33:Issue 1(2018)
• Page Start: 73
• Page End: 78
• Publication Date: 2018-01
• Subjects: Epithelial-to-mesenchymal transition -- Invasion -- IQGAP1 -- Laryngeal squamous cell carcinoma (LSCC); Cell receptors -- Periodicals; Histochemistry -- Periodicals; Tumor markers -- Periodicals; Tumor antigens -- Periodicals; 616.99407582
• DOI: 10.5301/ijbm.5000287
• Languages: English
• ISSNs: 0393-6155

Abstract

Background: Laryngeal squamous cell carcinoma (LSCC) has a poor prognosis due to recurrence and metastasis. IQ-domain GTPase-activating protein 1 (IQGAP1), a scaffold protein, plays an important role in tumorigenesis and malignant development. In this study, we aimed to explore the role of IQGAP1 in LSCC. Methods: Expression of IQGAP1 in human LSCC specimens was assessed by immunohistochemistry. We also evaluated the roles of IQGAP1 in cell proliferation, migration and invasion and epithelial-to-mesenchymal transition (EMT) in Hep-2 cells. Results: The expression of IQGAP1 protein was significantly up-regulated in LSCC tissues compared with normal laryngeal tissues (p = 0.002). Furthermore, the knockdown of IQGAP1 in Hep-2 cells inhibited cell growth, migration and invasion. Moreover, we found that IQGAP1 silencing reversed EMT. Conclusions: These results show for the first time that IQGAP1 is up-regulated in LSCC tissues and plays an important role in LSCC cell proliferation and invasiveness, which indicates that IQGAP1 could work as an oncogene and may serve as a promising molecular target for treatment of LSCC.