Biofilm formation in erythromycin-resistant Staphylococcus aureus and the relationship with antimicrobial susceptibility and molecular characteristics. (November 2018)

• Record Type: Journal Article
• Authors: Sun, Xiang; Lin, Zhi-wei; Hu, Xiao-xiong; Yao, Wei-ming; Bai, Bing; Wang, Hong-yan; Li, Duo-yun; Chen, Zhong; Cheng, Hang; Pan, Wei-guang; Deng, Ming-gui; Xu, Guang-jian; Tu, Hao-peng; Chen, Jun-wen; Deng, Qi-wen; Yu, Zhi-jian; Zheng, Jin-xin
• Is Part Of: Microbial pathogenesis. Volume 124(2018)
• Page Start: 47
• Page End: 53
• Publication Date: 2018-11
• Subjects: Erythromycin resistance -- Staphylococcus aureus -- Biofilm formation -- Methicillin-resistant Staphylococcus aureus -- Multilocus sequence typing -- Clonal complex; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.08.021
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Purpose: In this study, we aimed to investigate biofilm formation characteristics in clinical Staphylococcus aureus ( S. aureus ) isolates with erythromycin (ERY) resistance from China and further analyze their correlations with antimicrobial susceptibility and molecular characteristics. Methodology: A total of 276 clinical isolates of ERY-resistant S. aureus, including 142 methicillin-resistant S. aureus (MRSA) strains and 134 methicillin-susceptible S. aureus (MSSA) strains, were retrospectively collected in China. Biofilms were determined by crystal violet staining and ERY resistance genes ( ermA, ermB and ermC ) were detected by polymerase chain reaction. Inducible clindamycin resistance was examined by D test and multilocus sequence typing, and clonal complexes (CCs) based on housekeeping genes were further determined. Results: The frequency of biofilm formation among ERY-resistant S. aureus was 40.9% (113/276) in total and no significant difference was found for the frequency of biofilm formation between ERY-resistant MRSA and ERY-resistant MSSA (44.4% vs 37.3%, P > 0.05). In ERY-resistant MRSA isolates, the frequency of biofilm formation in ermA -positive, gentamicin-resistant and ciprofloxacin-resistant isolates was higher than that in ermA -negative, gentamicin-sensitive and ciprofloxacin-sensitive isolates, respectively (63.9% vs 23.6%, P < 0.01; 60.3% vs 27.5%, P < 0.01; 65.2% vs 26.3%, P < 0.01). In addition, tetracycline resistance facilitated biofilm formation in both ERY-resistant MRSA and MSSA and the frequency of biofilm formation in CC239- or CC7 S. aureus isolates with ERY resistance was significantly higher compared with that in CC59 S. aureus (both P < 0.01). Conclusion: The ermA gene, and gentamicin, ciprofloxacin and tetracycline resistance facilitate biofilm formation in ERY-resistant MRSA isolates and, moreover, ERY-resistant S. aureus isolates with positive biofilm formation exhibited clonality clustering regarding CC239 and CC7. Highlights: In ERY- resistant MRSA isolates, the frequency of biofilm formation in ermA-positive was higher than that in ermA-negative group. The CC239- or CC7- isolates formed more biofilms than that in CC59 isolates. Tetracycline resistance facilitate the biofilm formation in both ERY-resistant MRSA and MSSA. The Gentamicin -resistant or Ciprofloxacin-resistant isolates formed more biofilms than that in those sensitive isolates.