Protective effect of chlorogenic acid on lipopolysaccharide-induced inflammatory response in dairy mammary epithelial cells. (November 2018)

• Record Type: Journal Article
• Authors: Gao, Ruifeng; Yang, Huidi; Jing, Shangfei; Liu, Bo; Wei, Mao; He, Pengfei; Zhang, Naisheng
• Is Part Of: Microbial pathogenesis. Volume 124(2018)
• Page Start: 178
• Page End: 182
• Publication Date: 2018-11
• Subjects: Chlorogenic acid -- Bovine mastitis -- Mammary epithelial cell -- Proinflammatory cytokines; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.07.030
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Mastitis is a major disease of dairy cattle. Given the recent emergence of antibiotics resistance to mastitis, new intramammary treatments are urgently required. In the present study, we investigated whether lipopolysaccharide (LPS) could induce the increase in the proinflammatory cytokines in bovine mammary epithelial cells (MECs), and whether a natural antimicrobial compound Chlorogenic acid (CGA) could attenuate the inflammatory responses induced by LPS and thus could be a potential therapeutic compound for bovine mastitis. Our results indicated that LPS could induce the expression of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukine (IL)-1β and IL-6, and the activation of NF-κB p65 and p-p65 in primary bovine MECs. Furthermore, CGA significantly inhibited not only the protein expression of NF-κB p65 and p-p65 but also the mRNA expression of TNF-α, IL-1β and IL-6 after LPS treatment in primary bovine MECs. These results suggested that CGA had anti-inflammatory role by inhibiting NF-κB activation. In conclusion, CGA could be possibly used as a potential therapeutic compound for bovine mastitis. Highlights: The anti-inflammatory effects of Chlorogenic acid (CGA) were investigated in bovine mammary epithelial cells (MECs). Both proinflammatory cytokines and phosphorylation of NF-κB p65 were increased by LPS in bovine MECs. CGA clearly inhibited the expression of proinflammatory mediators such as, TNF-α, IL-1β and IL-6 in LPS-stimulated bovine MECs. CGA significantly decreased phosphorylation of NF-κB p65 in LPS-stimulated bovine MECs.