Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces against P. Mirabilis, C. Glabrata and C. Tropicalis Strains. Issue 4 (October 2015)

• Record Type: Journal Article
• Authors: Sahal, Gulcan; Nasseri, Behzad; Bilkay, Isil Seyis; Piskin, Erhan
• Is Part Of: Journal of applied biomaterials & functional materials. Volume 13:Issue 4(2015)
• Page Start: 351
• Page End: 355
• Publication Date: 2015-10
• Subjects: Biomaterial infections -- C. glabrata -- C. tropicalis -- Nanometer scale silver coatings -- P. mirabilis; Biomedical materials -- Periodicals; Bioengineering -- Periodicals; Biomechanics -- Periodicals; 610.28
• DOI: 10.5301/jabfm.5000248
• Languages: English
• ISSNs: 2280-8000

Abstract

Purpose: Nowadays, in order to terminate biofilm associated infections, coating of particular biomaterial surfaces with particular substances, via some nanotechnological tools, is being applied. Therefore, in the present study, investigation of anti-biofilm effects of nanometer scale silver (NmSAg) coatings on glass and polystyrene surfaces against clinical strains of Proteus mirabilis, Candida glabrata and Candida tropicalis was aimed. Methods: In this study, glass and polystyrene slabs with 1.5 cm × 1.5 cm × 0.3 mm dimensions were cleaned by using surface plasma technology, covered with NmSAg by using a physical vapor deposition machine, and biofilm inhibition was determined by crystal violet binding assay. Results: According to our results, 32 nm of silver layer on a glass slab decreased biofilm formation of P. mirabilis strain to a maximum amount of 88.1% and caused 20.9% inhibition in biofilm formation of C. glabrata strain. On the other hand, NmS coating of Ag on a polystyrene slab caused 34.4% and 20% inhibitions, respectively, in biofilm formations of C. glabrata and C. tropicalis strains. Although biofilm inhibition of NmSAg layer on polystyrene slab was more (34.4%) than biofilm inhibition caused by NmSAg layer on glass slab (20.9%), C. glabrata strain's biofilm formation on uncoated glass slab was lower than both uncoated and NmSAg-coated polystyrene slabs. Conclusions: Our results show that glass surfaces with NmSAg coatings can be used as a new surface material of various indwelling devices on which P. mirabilis colonizations frequently occur and in order to avoid C. glabrata -associated biofilm infections, it is more useful to choose a surface material of glass rather than choosing a surface material of polystyrene.