Efficacy of PCR-based open reading frame typing assay for outbreak investigation of metallo-β-lactamase-producing Pseudomonas aeruginosa in hematology unit. Issue 12 (December 2018)

• Record Type: Journal Article
• Authors: Nakamura, Ayako; Misawa, Shigeki; Chonan, Masayoshi; Kawakami, Takaaki; Horii, Takashi; Komatsu, Norio; Ohsaka, Akimichi
• Is Part Of: Journal of infection and chemotherapy. Volume 24:Issue 12(2018:Dec.)
• Page Start: 1020
• Page End: 1023
• Publication Date: 2018-12
• Subjects: Metallo-β-lactamase-producing Pseudomonas aeruginosa -- Outbreak -- PCR-based open reading frame typing (POT) -- Repetitive-sequence-based PCR typing; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2018.06.002
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: We investigated the efficacy of the PCR-based open reading frame typing (POT) assay for outbreak investigation of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MBL-PA). A total of 53 P . aeruginosa isolates were detected between January 2010 and December 2012 on a hematology ward, of which 6 were identified as MBL-PA with the bla IMP-1 gene. The POT assay revealed the same genotype (207-41) in 3 of 6 MBL-PA, suggesting an outbreak caused by a single strain. Environmental investigation of bathroom samples revealed the same POT genotype (207-41) as those of the clinical isolates and no other MBL-PA strains. Genetic relatedness of the MBL-PA isolates was confirmed by the DiversiLab repetitive-sequence-based PCR typing system, suggesting the POT type 207-41 as a genetically identical clone. The POT assay can be successfully applied to MBL-PA genotyping.