Antitumor activity of a molecularly imprinted nanopreparation of 5-flurouracil against Ehrlich's carcinoma solid tumors grown in mice: Comparison to free 5-flurouracil. (1st November 2018)

• Record Type: Journal Article
• Authors: Gardouh, Ahmed R.; Barakat, Bassant M.; Qushawy, Mona K.E.; El-kazzaz, Amany Y.; Sami, Manal M.; Zaitone, Sawsan A.
• Is Part Of: Chemico-biological interactions. Volume 295(2018)
• Page Start: 52
• Page End: 63
• Publication Date: 2018-11-01
• Subjects: Ehrlich's solid tumor -- 5-Flourouracil -- Molecularly-imprinted nanopreparation -- Mouse -- Precipitation polymerization; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2018.04.019
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Recently, nanotechnology has received great attention in war against cancer. The present study investigated the antitumor efficacy of molecularly imprinted nanopreparation of 5-fluorouracil (nano-5-FU) against Ehrlich ascites carcinoma (EAC) solid tumors grown in mice. Tumor cells were transplanted into female albino mice. Mice were allocated into 5 groups; Group 1: control EAC bearing mice. Groups 2&3: EAC-bearing mice treated orally with 5-FU (5 and 10 mg/kg) twice weekly. Groups 4&5: EAC bearing mice treated with nano-5-FU (5 and 10 mg/kg) twice weekly. Treatment with nano-5-FU showed higher antitumor effect compared to free 5-FU as indicated by enhanced apoptosis and reduction in tumor weight. Additionally, lower number of mitotic figures and greater area for necrosis were observed in the tumor specimens alongside with a decline in the number of intratumoral proliferating nuclei in comparison to free 5-FU. Furthermore, the results showed a significant down-regulation in tumoral expression of caspase-3 and vascular endothelial growth factor. Together, these results further support the potential of using nanotechnology to enhance anticancer efficacy of 5-FU. Graphical abstract: Highlights: Molecularly imprinted polymers can provide intelligent drug delivery device. 5-Fluorouracil is utilized as an antitumor drug for treating many types of tumors. 5-Fluorouracil as molecularly imprinted polymers showed greater antitumor activity. This polymeric nanopreparation reduced tumor proliferation and angiogenesis.