A tissue factor-cascade-targeted nanoparticle forsite-directed inducing thrombosis. (September 2017)

• Record Type: Journal Article
• Authors: Jin, Weiwei; Yin, Yanxue; Zhang, Bo; Mei, Heng; Wang, Huafang; Guo, Tao; Shi, Wei; Hu, Yu
• Is Part Of: Journal of biomaterials applications. Volume 32:Number 3(2017:Sep.)
• Page Start: 342
• Page End: 348
• Publication Date: 2017-09
• Subjects: Tissue factor -- thrombosis -- nanoparticles -- EGFP-EGF1 protein -- photochemistry -- HMME; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328217722740
• Languages: English
• ISSNs: 0885-3282

Abstract

Tissue factor is an upstream component of the cascade and a high-expressing factor under phathological conditions. In this study, a tissue factor cascade-targeted strategy for inducing local thrombosis was developed by combining ENP-HMME and photochemistry. In vitro study showed that protein EGFP-EGF1 conjugation to the nanoparticles could significantly contribute to the uptake of nanoparticles by tissue factor over-expressed brain capillary endothelial cells. Three-dimensional imaging and specklegram of brains in vivo showed that tissue factor cascade-targeted strategy successfully induced thrombosis of expected position. As shown by the in vivo multispectral fluorescent imaging, when ENP-HMME was combined with photochemistry, higher accumulation in the infarction hemisphere was observed, which might suggest that the photochemistry inducing tissue factor cascade recruited more ENP-HMME than HMME-loaded nanoparticles (NP-HMME). The data indicated the tissue factor cascade-targeted strategy has potential to induce local thrombosis, and might be applied in the treatment of a variety of hypervascular diseases.