Back Cover: Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor‐Binding Element of Amine Transaminase (ChemBioChem 15/2017). (19th July 2017)

• Record Type: Journal Article
• Authors: Börner, Tim; Rämisch, Sebastian; Bartsch, Sebastian; Vogel, Andreas; Adlercreutz, Patrick; Grey, Carl
• Is Part Of: Chembiochem. Volume 18:Number 15(2017)
• Page Start: 1558
• Page End: 1558
• Publication Date: 2017-07-19
• Subjects: amines -- enzyme catalysis -- operational stability -- pyridoxamine 5′-phosphate -- transaminase; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201700373
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : The back cover picture shows an engineered, highly stable amine transaminase that catalyses the enantioselective amination of ketones under process conditions, that is, high temperature and substrate concentration as well as solvent use. Through a semirational design, the two point mutations in the cofactor binding region (illustrated by the screws) prevent protein unfolding and cofactor loss (indicated by the chain) during catalysis. More information can be found in the communication by C. Grey et al. onpage 1482 in Issue 15, 2017 (DOI:10.1002/cbic.201700236 ).