Hydrolysis of sterol esters by an esterase from Ophiostoma piceae: application to pitch control in pulping of Eucalyptus globulus wood. (14th October 2004)

• Record Type: Journal Article
• Authors: Calero-Rueda, Olga; Gutierrez, Ana; Del Rio, Jose C.; Prieto, Alicia; Plou, Francisco; Ballesteros, Antonio; Martinez, Angel; Martinez, Maria
• Is Part Of: International journal of biotechnology. Volume 6:Number 4(2004)
• Page Start: 367
• Page End: 375
• Publication Date: 2004-10-14
• Subjects: sterol esterase -- wood extractives -- biocontrol -- fungus -- ascomycete -- kinetics -- biotechnology -- hydrolysis -- paper pulp manufacture; Biotechnology -- Economic aspects -- Periodicals; Biotechnology -- Research -- Periodicals; 338.47660605
• Languages: English
• ISSNs: 0963-6048

Abstract

A sterol esterase purified from cultures of the sapstain fungus Ophiostoma piceae was able to hydrolyse sterol esters and glycerides. The kinetics of sterol esters and triglyceride hydrolysis by this new esterase, estimated using a pH-stat, showed a Km app and a kcat app in the range of 0.9–1.1 mM and 70–300 s -1, respectively. Its ability to hydrolyse both pure sterol esters and natural mixtures of saponifiable lipids from eucalypt wood was compared with those of commercial sterol esterases from other microbial sources. Its specific activity on sterol esters was higher than that found with all the commercial esterases assayed, and the highest hydrolysis of eucalypt sterol esters was also attained using the O. piceae esterase. This sterol esterase could be of biotechnological interest for the hydrolysis of sterol esters that form pitch deposits in paper pulp manufacturing.