Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. (20th September 2015)

• Record Type: Journal Article
• Authors: Yuan, Yuan; Macquarrie, Duncan
• Is Part Of: Carbohydrate polymers. Volume 129(2015)
• Page Start: 101
• Page End: 107
• Publication Date: 2015-09-20
• Subjects: Sulfated polysaccharides -- Fucoidan -- Microwave assisted extraction -- Ascophyllum nodosum -- Antioxidant activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.04.057
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Microwave assisted extraction technology was used. A 3*3 h's conventional extraction was also compared. Ascophyllum nodosum has the potential as natural antioxidants. Mass balance of the whole extraction process was carried out. Abstract: Sulfated polysaccharides (fucoidan) from brown seaweed Ascophyllum nodosum were extracted by microwave assisted extraction (MAE) technology. Different conditions of temperature (90–150 °C), extraction time (5–30 min) were evaluated and optimal fucoidan yield was 16.08%, obtained from 120 °C for 15 min's extraction. Compositional analysis, GPC, HPAEC and IR analysis were employed for characterization of extracted sulfated polysaccharides. Fucose was the main monosaccharide of fucoidan extracted at 90 °C while glucuronic acid was the main monosaccharide of fucoidan extracted at 150 °C. Both the molecular weight and sulfate content of extracted fucoidan increased with decreasing extraction temperature. All fucoidans exhibited antioxidant activities as measured by DPPH scavenging and reducing power, among which fucoidan extracted at 90 °C was highest. This study shows that MAE is an efficient technology to extract sulfated polysaccharides from seaweed and Ascophyllum nodosum could potentially be a resource for natural antioxidants.