Fast kinetics of calcium signaling and sensor design. (August 2015)

• Record Type: Journal Article
• Authors: Tang, Shen; Reddish, Florence; Zhuo, You; Yang, Jenny J
• Is Part Of: Current opinion in chemical biology. Volume 27(2015)
• Page Start: 90
• Page End: 97
• Publication Date: 2015-08
• Subjects: Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2015.06.014
• Languages: English
• ISSNs: 1367-5931

Abstract

Highlights: Fast calcium signaling is mainly regulated by calcium channels. Calcium indicators facilitate our understanding of fast calcium signaling. It is necessary to improve the kinetics of genetically encoded calcium indicators. CatchER with a pentagonal bipyramidal-like binding domain exhibits fast kinetics. Fluorescent lifetime calcium imaging is advantageous for deep field imaging. Abstract : Fast calcium signaling is regulated by numerous calcium channels exhibiting high spatiotemporal profiles which are currently measured by fluorescent calcium sensors. There is still a strong need to improve the kinetics of genetically encoded calcium indicators (sensors) to capture calcium dynamics in the millisecond time frame. In this review, we summarize several major fast calcium signaling pathways and discuss the recent developments and application of genetically encoded calcium indicators to detect these pathways. A new class of genetically encoded calcium indicators designed with site-directed mutagenesis on the surface of beta-barrel fluorescent proteins to form a pentagonal bipyramidal-like calcium binding domain dramatically accelerates calcium binding kinetics. Furthermore, novel genetically encoded calcium indicators with significantly increased fluorescent lifetime change are advantageous in deep-field imaging with high light-scattering and notable morphology change.