B- and T-cell epitope mapping of human sapovirus capsid protein: an immunomics approach. (3rd August 2011)

• Record Type: Journal Article
• Authors: Amin, M. Ruhul; Siddiqui, Mohammad S.; Ahmed, Dilruba; Ahmed, Firoz; Hossain, Anowar
• Is Part Of: International journal of bioinformatics research and applications. Volume 7:Number 3(2011)
• Page Start: 287
• Page End: 298
• Publication Date: 2011-08-03
• Subjects: sapovirus -- capsid protein -- vaccine -- homology modelling -- epitope mapping; Bioinformatics -- Periodicals; 570.285
• Languages: English
• ISSNs: 1744-5485

Abstract

Human sapovirus is one of the major causes of viral gastroenteritis. Although the capsid protein (VP1) confers antigenic cross-reactivity, immunity against sapovirus is still unclear. Using immunoinformatics approach, we defined putative T- and B-cell epitopes of VP1 and mapped on to its predicted three-dimensional structure. Identified five putative T-cell epitopes also occupied the putative B-cell epitope region. These putative epitopes were conserved in all existing serotypes. Predicted epitopes can be generated through proteasome cleavage and may be useful in designing peptide-based subunit vaccine to confer both humoral and cell-mediated immunity.