Morphology visualization of irregular shape bacteria by electron holography and tomography. Issue 12 (11th August 2017)

• Record Type: Journal Article
• Authors: Ortega, Eduardo; Cantu‐Valle, Jesús; Plascencia‐Villa, Germán; Vergara, Sandra; Mendoza‐Santoyo, Fernando; Londono‐Calderon, Alejandra; Santiago, Ulises; Ponce, Arturo
• Is Part Of: Microscopy research and technique. Volume 80:Issue 12(2017)
• Page Start: 1249
• Page End: 1255
• Publication Date: 2017-08-11
• Subjects: 3D visualization -- electron holography -- electron tomography -- S. aureus bacteria; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22923
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: In the current work, irregular morphology of Staphylococcus aureus bacteria has been visualized by phase retrieval employing off‐axis electron holography (EH) and 3D reconstruction electron tomography using high‐angle annular dark field scanning transmission electron microscopy (HAADF‐STEM). Bacteria interacting with gold nanoparticles (AuNP) acquired a shrunken or irregular shape due to air dehydration processing. STEM imaging shows the attachment of AuNP on the surface of cells and suggests an irregular 3D morphology of the specimen. The phase reconstruction demonstrates that off‐axis electron holography can reveal with a single hologram the morphology of the specimen and the distribution of the functionalized AuNPs. In addition, EH reduces significantly the acquisition time and the cumulative radiation damage (in three orders of magnitude) over biological samples in comparison with multiple tilted electron expositions intrinsic to electron tomography, as well as the processing time and the reconstruction artifacts that may arise during tomogram reconstruction. Abstract : Off‐axis electron holography and electron tomography have been used to show an irregular shape of S. aureus bacteria. EH reduces acquisition and processing time as well as the radiation damage inherent to the long tilting series for tomography.