Effects of ω-3 Fatty Acids on Toll-like Receptor 4 and Nuclear Factor κB p56 in the Pancreas of Rats With Severe Acute Pancreatitis. Issue 10 (November 2017)

• Record Type: Journal Article
• Authors: Wang, Bin; Xu, Xiao-Bing; Jin, Xin-Xin; Wu, Xiao-Wei; Li, Min-Li; Guo, Mei-Xia; Zhang, Xiao-Hua
• Is Part Of: Pancreas. Volume 46:Issue 10(2017)
• Publication Date: 2017-11
• Subjects: ω-3 fatty acids -- fish oil -- NF-κBp56 -- cytokine -- severe acute pancreatitis -- Toll-like receptor 4; Pancreas -- Diseases -- Periodicals; Pancreas -- Periodicals; Neuroendocrine tumors -- Periodicals; 616.37005
• DOI: 10.1097/MPA.0000000000000935
• Languages: English
• ISSNs: 0885-3177

Abstract

Abstract : Objectives: The aims of this study were to determine the effects of ω-3 fatty acids (ω-3FAs) on the Toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signaling pathway in the pancreas of rats with severe acute pancreatitis (SAP). Methods: Sixty-four Sprague-Dawley rats were randomly divided into 4 groups: the control, SAP-saline, SAP–soybean oil, and SAP–ω-FA groups. Severe acute pancreatitis was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the pancreas was evaluated by immunohistochemistry and Western blot analysis. The levels of the proinflammatory cytokines interleukin 6 and tumor necrosis factor α in the pancreas were measured by enzyme-linked immunosorbent assays. Results: Toll-like receptor 4, NF-κBp56, and inflammatory cytokine expression in the pancreas was increased significantly in the SAP group compared with that in the control group ( P < 0.05), but was significantly decreased in the ω-3FA group compared with that in the soybean oil group at 24 and 48 hours ( P < 0.05). Conclusions: Our results suggest that during the initial stage of SAP ω-3FAs could efficiently lower the inflammatory response by activating the TLR4/NF-κBp56 signaling pathway.