Biphasic Renal Sympathetic Response to Hemorrhagic Hypotension in Mice. Issue 5 (November 2017)

• Record Type: Journal Article
• Authors: Zhang, Tao; Tanida, Mamoru; Uchida, Kunitoshi; Suzuki, Yoshiro; Yang, Wei; Kuda, Yuhichi; Kurata, Yasutaka; Tominaga, Makoto; Shibamoto, Toshishige
• Is Part Of: Shock. Volume 48:Issue 5(2017)
• Publication Date: 2017-11
• Subjects: Autonomic nerves -- hemorrhage -- knockout mice -- TRPV1 -- vagotomy; Shock -- Periodicals; Shock -- Periodicals; Choc (Pathologie) -- Périodiques; Shock; Periodicals; 616.0475
• DOI: 10.1097/SHK.0000000000000889
• Languages: English
• ISSNs: 1073-2322

Abstract

ABSTRACT: Aim: The inhibitory responses of renal sympathetic nerve activity (RSNA) and heart rate (HR) to sustained hemorrhagic shock occurred in anesthetized rats, but have not yet been determined in mice. Here, we investigated the responses of RSNA and HR to hemorrhagic hypotension in anesthetized mice, with an emphasis on the molecule-based mechanism for roles of afferent vagal nerves. Methods: RSNA, HR, and mean systemic arterial pressure were continuously measured in male pentobarbital-anesthetized C57BL/6N mice. Hemorrhagic hypotension of 50 mmHg was evoked and maintained for 10 min. Results: During hemorrhagic hypotension, RSNA initially increased and then sustainedly decreased, while HR progressively decreased. Vagotomy eliminated the second-phase sympathoinhibition and bradycardia, and carotid sinus denervation with vagotomy abolished the initial renal sympathoexcitation. The renal sympathoinihibition during hemorrhagic hypotension of 50 mmHg was eliminated in mice pretreated with a transient receptor potential vanilloid type 1 channel (TRPV1) inhibitor, capsazepine, and in TRPV1 knockout (TRPV1 −/− ) mice, but not in TRPV4 knockout mice. The bradycardia response to hemorrhagic hypotension was also absent in TRPV1 −/− mice and mice pretreated with capsazepine. Conclusion: Hemorrhagic hypotension in anesthetized mice causes biphasic responses of RSNA with an initial increase, followed by a sustained decrease, and a progressive decrease in HR. The initial sympathoexcitation is mediated by carotid sinus baroreceptors, while the later sympathoinhibition and bradycardia are mediated via the TRPV1 signals of vagal afferents.