DNA Triplexes That Bind Several Cofactor Molecules. Issue 51 (12th November 2015)

• Record Type: Journal Article
• Authors: Vollmer, Sven; Richert, Clemens
• Is Part Of: Chemistry. Volume 21:Issue 51(2015)
• Page Start: 18613
• Page End: 18622
• Publication Date: 2015-11-12
• Subjects: cofactors -- DNA -- oligonucleotides -- nucleotide storage -- triplexes; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201503220
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Cofactors are critical for energy‐consuming processes in the cell. Harnessing such processes for practical applications requires control over the concentration of cofactors. We have recently shown that DNA triplex motifs with a designed binding site can be used to capture and release nucleotides with low micromolar dissociation constants. In order to increase the storage capacity of such triplex motifs, we have explored the limits of ligand binding through designed cavities in the oligopurine tract. Oligonucleotides with up to six non‐nucleotide bridges between purines were synthesized and their ability to bind ATP, cAMP or FAD was measured. Triplex motifs with several single‐nucleotide binding sites were found to bind purines more tightly than triplexes with one large binding site. The optimized triplex consists of 59 residues and four C3‐bridges. It can bind up to four equivalents of ligand with apparent K d values of 52 µM for ATP, 9 µM for FAD, and 2 µM for cAMP. An immobilized version fuels bioluminescence via release of ATP at body temperature. These results show that motifs for high‐density capture, storage and release of energy‐rich biomolecules can be constructed from synthetic DNA. Abstract : DNA as storage material : DNA triplex motifs with a designed binding site can be used to capture and release nucleotides with low micromolar dissociation constants. In order to increase the storage capacity of such triplex motifs, the limits of ligand binding through designed cavities in the oligopurine tract have been explored. Oligonucleotides with up to six non‐nucleotide bridges between purines were synthesized and their ability to bind ATP, cAMP or FAD was measured.