Remote Regulation of Membrane Channel Activity by Site‐Specific Localization of Lanthanide‐Doped Upconversion Nanocrystals. Issue 11 (3rd February 2017)

• Record Type: Journal Article
• Authors: Ai, Xiangzhao; Lyu, Linna; Zhang, Yang; Tang, Yanxia; Mu, Jing; Liu, Fang; Zhou, Yixi; Zuo, Zhenghong; Liu, Gang; Xing, Bengang
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 11(2017)
• Page Start: 3031
• Page End: 3035
• Publication Date: 2017-02-03
• Subjects: click chemistry -- ion channels -- lanthanides -- nanocrystals -- upconversion; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201612142
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The spatiotemporal regulation of light‐gated ion channels is a powerful tool to study physiological pathways and develop personalized theranostic modalities. So far, most existing light‐gated channels are limited by their action spectra in the ultraviolet (UV) or visible region. Simple and innovative strategies for the specific attachment of photoswitches on the cell surface without modifying or genetically encoding channel structures, and more importantly, that enable the remote activation of ion‐channel functions within near‐infrared (NIR) spectral window in living systems, remain a challenging concern. Herein, metabolic glycan biosynthesis is used to achieve site‐specific covalent attachment of near‐infrared‐light‐mediated lanthanide‐doped upconversion nanocrystals (UCNs) to the cell surface through copper‐free click cyclization. Upon irradiation with 808 nm light, the converted emission at 480 nm could activate a light‐gated ion channel, channelrhodopsins‐2 (ChR2), and thus remotely control the cation influx. This unique strategy provides valuable insights on the specific regulation membrane‐associated activities in vivo. Abstract : Shine on : Near‐infrared (NIR)‐light‐mediated lanthanide‐doped upconversion nanocrystals (UCNs) can be site‐specifically covalently localized onto the cell membrane. Upon excitation (Ex), the upconverted blue light emission (Em) can effectively activate the light‐gated ion channel. In this way, cation influx can be controlled and the ion‐mediated signaling of apoptosis in living cells and zebrafish manipulated.