Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers. (October 2017)

• Record Type: Journal Article
• Authors: Song, Zhihua; Cheng, Kang; Zhang, Lili; Wang, Tian
• Is Part Of: Journal of thermal biology. Volume 69(2017)
• Page Start: 184
• Page End: 190
• Publication Date: 2017-10
• Subjects: Enzymatically treated Artemisia annua -- Inflammatory response -- Intestine -- Heat stress -- Broilers; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2017.07.015
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: The study was undertaken to evaluate the effects of enzymatically treated Artemisia annua (EA) on the intestinal inflammatory response of heat-stressed broilers. A total of 144 male Arbor Acres broilers aged 21 days were randomly divided into 3 groups with 6 replicates per group and 8 broilers in each replicate. The 3 treatment groups were as follows: the control group, in which broiler chickens were raised at 22 ± 1 °C and fed basal diets, the heat stress (HS) and HS-EA groups, in which broiler chickens were raised at 34 ± 1 °C for 8 h (0900–1700 h) and 22 ± 1 °C for 16 h, and fed basal diets supplemented with 0 or 1 g/kg EA, respectively. From 22 to 41 days, the heat treatment lasted for 20 consecutive days. Compared with the control group, HS increased the activity of plasma diamine oxidase ( P < 0.05) and the concentration of intestinal interleukin-1β, and up-regulated ( P < 0.05) the mRNA expression of intestinal interleukin-1β, interleukin-6, interferon-γ, toll-like receptor 4 and heat shock protein 70, down-regulated ( P < 0.05) jejunal zonula occludens-1 and ileal occluding mRNA abundances and intestinal interleukin-10 at both protein and transcriptional levels. However, EA treatment significantly decreased ( P < 0.05) plasma diamine oxidase activity, the mRNA expression of heat shock protein 70, toll-like receptor 4, interleukin-6, interleukin-1β and interferon-γ in intestine of heat-stressed broilers, whereas upregulated ( P < 0.05) the mRNA expression of ileal occluding, jejunal zonula occludens-1 and occluding. In addition, both protein and transcriptional levels of interleukin-10 in jejunum and ileum were increased ( P < 0.05) by EA treatment in the HS group. In conclusion, dietary EA supplementation could alleviate the intestinal inflammatory response, and improve the intestinal barrier function in broilers during the heat stress period. Highlights: The increased mRNA abundance of interleukin-1β, interleukin-4, interleukin-7, interferon-γ, toll-like receptor 4, and heat shock protein 70 in jejunum and ileum induced by heat stress were counteracted by EA supplementation. EA treatment increased the ileal occluding, jejunal zonula occludens-1 and occluding mRNA expression, interleukin-10 at both protein and transcriptional levels in jejunum and ileum of broilers exposed to heat stress. EA could alleviate inflammatory response and protect intestinal integrity in heat-stressed broilers.