The Microbiome in Posttraumatic Stress Disorder and Trauma-Exposed Controls: An Exploratory Study. Issue 8 (October 2017)

• Record Type: Journal Article
• Authors: Hemmings, Sian M.J.; Malan-Müller, Stefanie; van den Heuvel, Leigh L.; Demmitt, Brittany A.; Stanislawski, Maggie A.; Smith, David G.; Bohr, Adam D.; Stamper, Christopher E.; Hyde, Embriette R.; Morton, James T.; Marotz, Clarisse A.; Siebler, Philip H.; Braspenning, Maarten; Van Criekinge, Wim; Hoisington, Andrew J.; Brenner, Lisa A.; Postolache, Teodor T.; McQueen, Matthew B.; Krauter, Kenneth S.; Knight, Rob; Seedat, Soraya; Lowry, Christopher A.
• Is Part Of: Psychosomatic medicine. Volume 79:Issue 8(2017)
• Publication Date: 2017-10
• Subjects: childhood trauma -- C-reactive protein -- immunoregulation -- inflammation -- microbiome -- posttraumatic stress disorder -- BMI = body mass index -- CAPS-5 = Clinician-Administered Posttraumatic Stress Disorder Scale for DSM-5 -- CRP = C-reactive protein -- CTQ = Childhood Trauma Questionnaire -- DSM-5 = Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition -- IBD = inflammatory bowel disease -- IBS = irritable bowel syndrome -- IgA = immunoglobulin A -- IL = interleukin -- IQR = interquartile range -- LEC-5 = Life Events Checklist for DSM-5 -- MDD = major depressive disorder -- OTUs = operational taxonomic units -- PTSD = posttraumatic stress disorder -- QIIME = Quantitative Insights into Microbial Ecology -- SA = South Africa -- TE = trauma-exposed -- Treg = regulatory T cell -- VSURF = variable selection using random forests; Medicine, Psychosomatic -- Periodicals; 616.0805
• DOI: 10.1097/PSY.0000000000000512
• Languages: English
• ISSNs: 0033-3174

Abstract

ABSTRACT: Objective: Inadequate immunoregulation and elevated inflammation may be risk factors for posttraumatic stress disorder (PTSD), and microbial inputs are important determinants of immunoregulation; however, the association between the gut microbiota and PTSD is unknown. This study investigated the gut microbiome in a South African sample of PTSD-affected individuals and trauma-exposed (TE) controls to identify potential differences in microbial diversity or microbial community structure. Methods: The Clinician-Administered PTSD Scale for DSM-5 was used to diagnose PTSD according to Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition criteria. Microbial DNA was extracted from stool samples obtained from 18 individuals with PTSD and 12 TE control participants. Bacterial 16S ribosomal RNA gene V3/V4 amplicons were generated and sequenced. Microbial community structure, α-diversity, and β-diversity were analyzed; random forest analysis was used to identify associations between bacterial taxa and PTSD. Results: There were no differences between PTSD and TE control groups in α- or β-diversity measures (e.g., α-diversity: Shannon index, t = 0.386, p = .70; β-diversity, on the basis of analysis of similarities: Bray-Curtis test statistic = –0.033, p = .70); however, random forest analysis highlighted three phyla as important to distinguish PTSD status: Actinobacteria, Lentisphaerae, and Verrucomicrobia. Decreased total abundance of these taxa was associated with higher Clinician-Administered PTSD Scale scores ( r = –0.387, p = .035). Conclusions: In this exploratory study, measures of overall microbial diversity were similar among individuals with PTSD and TE controls; however, decreased total abundance of Actinobacteria, Lentisphaerae, and Verrucomicrobia was associated with PTSD status. Abstract : Supplemental digital content is available in the text.