Enhanced Biological Hydrogen Production from Escherichia coli with Surface Precipitated Cadmium Sulfide Nanoparticles. Issue 20 (14th July 2017)

• Record Type: Journal Article
• Authors: Wang, Bo; Zeng, Cuiping; Chu, Ka Him; Wu, Dan; Yip, Ho Yin; Ye, Liqun; Wong, Po Keung
• Is Part Of: Advanced energy materials. Volume 7:Issue 20(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-07-14
• Subjects: cadmium sulfide -- Escherichia coli -- hybrid system -- hydrogen production -- natural resources; Energy harvesting -- Materials -- Periodicals; Energy conversion -- Materials -- Periodicals; Energy storage -- Materials -- Periodicals; Photovoltaics -- Periodicals; Fuel cells -- Periodicals; Thermoelectric materials -- Periodicals; 621.31
• DOI: 10.1002/aenm.201700611
• Languages: English
• ISSNs: 1614-6832

Abstract

Abstract: The precipitation of cadmium sulfide nanoparticles is induced on the surface of Escherichia coli, and the biological hydrogen production efficiency under visible light (VL) irradiation is investigated. When endogenous [Ni–Fe]‐hydrogenase is anaerobically induced, an additional 400 µmol of hydrogen gas is generated within 3 h from the hybrid system suspension (50 mL) under VL irradiation (2000 W m −2 ), corresponding to an increase in hydrogen production of ≈30%. The apparent quantum efficiencies of the hybrid system under 470 and 620 nm VL irradiation are 7.93% and 9.59%, respectively, which are higher than those of many photoheterotrophic bacteria. Furthermore, the mechanism of the enhanced hydrogen evolution is investigated. The interaction between photogenerated electrons and cells of E. coli is confirmed by heat‐treatment, electron‐scavenger, and separation studies. The acceleration of pyruvate generation, inhibition of lactate fermentation, increase of formate concentration, stimulation of hydrogenase activity, and elevation of nicotinamide adenine dinucleotide (NAD)H/NAD ratio in the hybrid system are responsible for the enhanced hydrogen production. A feasibility study is also conducted using wastewater and natural sunlight for the hydrogen production by the hybrid system. An additional 120 µmol of hydrogen is generated from the hybrid system within 3 h under these conditions using natural resources. Abstract : Surface precipitated cadmium sulfide nanoparticles can empower the Escherichia coli to make use of photogenerated electrons, leading to ≈30% enhancement in biological hydrogen generation under visible light irradiation. The photogenerated electrons can accelerate pyruvate generation, inhibit lactate fermentation, and increase formate concentration, hydrogenase activity, and NADH/NAD ratio. Natural resources can also be used for hydrogen production.