Histamine Downregulates Aquaporin 5 in Human Nasal Epithelial Cells. Issue 3 (May 2015)

• Record Type: Journal Article
• Authors: Wang, Weiwei; Wang, Xiaolian; Ma, Lan; Zhang, Ruitao
• Is Part Of: American journal of rhinology & allergy. Volume 29:Issue 3(2015)
• Page Start: 188
• Page End: 192
• Publication Date: 2015-05
• Subjects: Nose -- Periodicals; Allergy -- Periodicals; 616.21005
• DOI: 10.2500/ajra.2015.29.4181
• Languages: English
• ISSNs: 1945-8924

Abstract

Background: Aquaporin 5 (AQP5) is a water-specific channel protein. It is thought to be a key participant in fluid secretion and a rate-limiting barrier to the secretion seen during allergic inflammation. We sought to determine the effect of histamine on AQP5 expression in human nasal epithelial cells (HNEpC). Methods: HNEpC cells were cultured with four concentrations of histamine in vitro. The phosphorylation of cyclic adenosine monophosphate-responsive element binding protein (CREB) at serine 133 and the AQP5 protein were measured by using immunocytochemistry and Western blotting. Real-time polymerase chain reaction was used to detect AQP5 messenger ribonucleic acid (mRNA). Results: Concentration-dependent histamine induced-inhibition of CREB phosphorylation at serine 133 in HNEpC cells was observed, and AQP5 mRNA and protein were also downregulated in a concentration-dependent fashion. Conclusion: Histamine downregulates AQP5 production in HNEpC cells by inhibiting CREB phosphorylation at serine 133.