Implantable vaccine development using in vitro antigen-pulsed macrophages absorbed on laser micro-structured Si scaffolds. Issue 27 (17th June 2015)

• Record Type: Journal Article
• Authors: Zerva, Ioanna; Simitzi, Chara; Siakouli-Galanopoulou, Alexandra; Ranella, Anthi; Stratakis, Emmanuel; Fotakis, Costas; Athanassakis, Irene
• Is Part Of: Vaccine. Volume 33:Issue 27(2015)
• Page Start: 3142
• Page End: 3149
• Publication Date: 2015-06-17
• Subjects: Silicon scaffold -- Laser manufacturing -- Immune response -- Macrophage -- Scaffold implantation -- Vaccination; HSA human serum albumin -- FCA Freund complete adjuvant -- SEM scanning electron microscopy -- WBC white blood cells; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2015.04.017
• Languages: English
• ISSNs: 0264-410X

Abstract

Graphical abstract: Abstract: To overcome the limiting antigenic repertoire of protein sub-units and the side effects of adjuvants applied in second generation vaccines, the present work combined in vitro and in vivo manipulations to develop biomaterials allowing natural antigen-loading and presentation in vitro and further activation of the immune response in vivo. 3-dimensional laser micro-textured implantable Si-scaffolds supported mouse macrophage adherence, allowed natural seeding with human serum albumin (antigen) and specific antibody and inflammatory cytokine production in vitro . Implantation of Si-scaffolds loaded with antigen-activated macrophages induced an inflammatory reaction along with antigen-specific antibody production in vivo, which could be detected even 30 days post implantation. Analysis of implant histology using scanning electron microscopy showed that Si-scaffolds could be stable for a 6-month period. Such technology leads to personalized implantable vaccines, opening novel areas of research and treatment.