Determination and speciation of cadmium in microcosms with Bunodosoma caissarum and Perna perna using isotopically enriched 116Cd. (15th February 2017)

• Record Type: Journal Article
• Authors: Rizzini Ansari, Nafisa; Fernández Iglesias, Nerea; Cordeiro, Renato Campello; Fernandez, Marcos Antônio; Bettmer, Jörg
• Is Part Of: Marine pollution bulletin. Volume 115:Number 1/2(2017)
• Page Start: 362
• Page End: 368
• Publication Date: 2017-02-15
• Subjects: Inductively coupled plasma-mass spectrometry (ICP-MS) -- Isotope dilution analysis (IDA) -- Isotope pattern deconvolution (IPD) -- Cadmium -- Bunodosoma caissarum -- Perna perna -- Elemental speciation; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2016.12.010
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: The study of the uptake and distribution of elements in marine environments is of great interest for understanding their pathways and accumulation. Here, we investigated in laboratory experiments the accumulation behavior of Cd in the sea anemone Bunodosoma caissarum and the mussel Perna perna . Specimens were incubated with isotopically enriched 116 Cd in aquariums. Cd concentrations in the seawater and in the tissues of B . caissarum and P . perna were followed by inductively coupled plasma-mass spectrometry (ICP-MS) by means of isotope dilution analysis. Bioconcentration factors for B . caissarum and P . perna exposed to 0.9 μg·L − 1 of 116 Cd were determined to be 80.5 and 850, respectively. P . perna specimens exposed to 4.5 μg·L − 1 of 116 Cd reached 530. Cytosolic proteins associated with Cd from the tissues were extracted and further analyzed by size-exclusion chromatography coupled to ICP-MS. Cd accumulation could be detected in both organisms ranging from high-molecular to low-molecular species. Graphical abstract: Highlights: 116 Cd appeared to be a suitable tool to follow Cd uptake by B . caissarum and P . perna . P . perna showed a significant higher BCF than B . caissarum . Determined isotope ratios showed that 116 Cd uptake was different in the two species. Results suggest that mucus secretion of B . caissarum might support Cd excretion.