Screening of Polish Fir Honeydew Honey Using GC/MS, HPLC‐DAD, and Physical‐Chemical Parameters: Benzene Derivatives and Terpenes as Chemical Markers. Issue 9 (17th August 2017)

• Record Type: Journal Article
• Authors: Kuś, Piotr M.; Jerković, Igor; Marijanović, Zvonimir; Tuberoso, Carlo I. G.
• Is Part Of: Chemistry & biodiversity. Volume 14:Issue 9(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-08-17
• Subjects: Volatiles -- Antioxidants -- Lignin derivatives -- Terpenoids -- Bioactive compounds; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Biodiversity -- Periodicals; 572
• DOI: 10.1002/cbdv.201700179
• Languages: English
• ISSNs: 1612-1872

Abstract

Abstract : GC/MS of headspace solid phase micro extraction (HS‐SPME) and solvent extractives along with targeted HPLC‐DAD of Polish fir ( Abies alba Mill .) honeydew honey (FHH), were used to determine the chemical profiles and potential markers of botanical origin. Additionally, typical physical‐chemical parameters were also assigned. The values determined for FHH were: conductivity (1.2 mS/cm), water content (16.7 g/100 g), pH (4.5), and CIE chromaticity coordinates ( L * = 48.4, a * = 20.6, b * = 69.7, C * = 72.9, and h ° = 73.5). FHH contained moderate‐high total phenolic content (533.2 mg GAE/kg) and antioxidant activity (1.1 mmol TEAC/kg) and (3.2 mmol Fe 2+ /kg) in DPPH and FRAP assays. The chemical profiles were dominated by source plant‐originated benzene derivatives: 3, 4‐dihydroxybenzoic acid (up to 8.7 mg/kg, HPLC/honey solution), methyl syringate (up to 14.5%, GC/solvent extracts) or benzaldehyde (up to 43.7%, GC/headspace). Other markers were terpenes including norisoprenoid (4‐hydroxy‐3, 5, 6‐trimethyl‐4‐(3‐oxobut‐1‐enyl)cyclohex‐2‐en‐1‐one, up to 20.3%, GC/solvent extracts) and monoterpenes, mainly linalool derivatives (up to 49%, GC/headspace) as well as borneol (up to 5.9%, GC/headspace). The application of various techniques allowed comprehensive characterisation of FHH. 4‐Hydroxy‐3, 5, 6‐trimethyl‐4‐(3‐oxobut‐1‐enyl)cyclohex‐2‐en‐1‐one, coniferyl alcohol, borneol, and benzaldehyde were first time proposed for FHH screening. Protocatechuic acid may be a potential marker of FFH regardless of the geographical origin. Abstract :