Cytokinin Response Factor 5 has transcriptional activity governed by its C-terminal domain. (1st February 2017)

• Record Type: Journal Article
• Authors: Striberny, Bernd; Melton, Anthony E.; Schwacke, Rainer; Krause, Kirsten; Fischer, Karsten; Goertzen, Leslie R.; Rashotte, Aaron M.
• Is Part Of: Plant signaling & behavior. Volume 12:Number 2(2017)
• Publication Date: 2017-02-01
• Subjects: AP2 transcription factors -- arabidopsis thaliana -- cytokinin response factors -- transactivation domain; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2016.1276684
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Cytokinin Response Factors (CRFs) are AP2/ERF transcription factors involved in cytokinin signal transduction. CRF proteins consist of a N-terminal dimerization domain (CRF domain), an AP2 DNA-binding domain, and a clade-specific C-terminal region of unknown function. Using a series of sequential deletions in yeast-2-hybrid assays, we provide evidence that the C-terminal region of Arabidopsis CRF5 can confer transactivation activity. Although comparative analyses identified evolutionarily conserved protein sequence within the C-terminal region, deletion experiments suggest that this transactivation domain has a partially redundant modular structure required for activation of target gene transcription.