Ocular delivery of cyanidin-3-glycoside in liposomes and its prevention of selenite-induced oxidative stress. (2nd April 2016)

• Record Type: Journal Article
• Authors: Zhang, Jing; Liang, Xinli; Li, Xiang; Guan, Zhiyu; Liao, Zhenggen; Luo, Yun; Luo, Yunxia
• Is Part Of: Drug development and industrial pharmacy. Volume 42:Number 4(2016:Apr.)
• Page Start: 546
• Page End: 553
• Publication Date: 2016-04-02
• Subjects: Cyanidin-3-glycoside -- N-trimethyl chitosan -- oxidative stress -- precorneal residence time -- transcorneal permeability; Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.3109/03639045.2015.1088867
• Languages: English
• ISSNs: 0363-9045

Abstract

Abstract: Context : Cataracts have become the leading cause of blindness around the world, which is mainly mediated by oxidative stress. Objective : N-trimethyl chitosan (TMC)-coated liposomes of cyanidin-3-glycoside (C3G) (C3G-TCL) were prepared to attenuate oxidative stress induced by selenite sodium in rats. Materials and methods : C3G-TCL were prepared by reverse-phase evaporation method and then coated with self-synthesized TMC. The physicochemical properties were determined. A gamma-scintigraphy study was employed to evaluate the precorneal elimination of the radioactive preparations. The transcorneal visualization for fluorescence-labeled samples was determined by confocal laser scanning microscopy (CLSM). The in vivo anti-oxidative study using C3G-TCL was carried out in rats with selenite-induced cataracts by topical administration. Results : The sphere-like morphological characterization of the vesicles was confirmed by TEM, with a size of 158.3 ± 2.8 nm and a zeta potential of 31.7 mV. The encapsulation efficiency was (53.7 ± 0.2) % as measured by ultrafiltration. C3G-TCL showed a 3.3-fold increment in precorneal residence time when compared with that of the 99m Tc-solution. A TMC coating enhanced the transepithelial transport of liposomes to a depth of 40-μm in the cornea. Moreover, C3G-TCL could significantly elevate the activity of superoxide dismutase and catalase in lens and also show a considerable reversal of reduced glutathione activity. The lipid peroxidation in lens was strongly prevented when compared with that of groups treated with uncoated C3G-loaded liposomes. Discussion and conclusion : The coating material TMC for liposomes helps improve the anti-oxidative effect of C3G in vivo through prolonged residence time on the cornea and improved permeability in the corneal epithelium.