Metabolic inflexibility in individuals with obesity assessed by near-infrared spectroscopy. (November 2017)

• Record Type: Journal Article
• Authors: Soares, Rogério Nogueira; Reimer, Raylene A; Doyle-Baker, Patricia K; Murias, Juan M
• Is Part Of: Diabetes & vascular disease research. Volume 14:Number 6(2017:Nov.)
• Page Start: 502
• Page End: 509
• Publication Date: 2017-11
• Subjects: Glucose -- glucose metabolism -- glucose oxidation -- obesity -- metabolic diseases; Diabetic angiopathies -- Periodicals; 616.462005
• DOI: 10.1177/1479164117725478
• Languages: English
• ISSNs: 1479-1641

Abstract

Objective: To non-invasively evaluate differences in oxidative metabolism in individuals with obesity compared to normal weight using the near-infrared spectroscopy and vascular occlusion technique during hyperglycaemia. Methods: In all, 16 normal-weight individuals (body mass index: 21.3 ± 1.7 kg/m 2 ) and 13 individuals with obesity (body mass index: 34.4 ± 2.0 kg/m 2 ) had five vascular occlusion tests (pre, 30, 60, 90 and 120 min after glucose ingestion). Oxygen utilization was estimated from the area under the curve of the deoxyhemoglobin [HHb] signal during occlusion. Muscle reperfusion was derived from the area above the curve after cuff release. Results: The deoxyhemoglobin area under the curve during occlusion of the normal-weight individuals increased from 15, 732 ± 2344 (% . s) at pre to 18, 930 ± 3226 (% . s) ( p < 0.05) at 90 min after glucose ingestion. The deoxyhemoglobin area under the curve during occlusion decreased significantly from 14, 695 ± 3341 (% . s) at pre to 11, 273 ± 1825 (% . s) ( p < 0.05) and 11, 360 ± 1750 (% . s) ( p < 0.05) at 30 and 60 min, respectively, after glucose ingestion. The area above the curve of deoxyhemoglobin during reperfusion decreased significantly from 6450 ± 765 (% . s) at pre to 4830 ± 963 (% . s) ( p < 0.05) at 60 min and to 4210 ± 595 (% . s) ( p < 0.01) at 90 min in normal-weight individuals after glucose ingestion, with no changes observed in individuals with obesity. Conclusion: This study confirmed in vivo and non-invasively the metabolic inflexibility of skeletal muscle in individuals with obesity during hyperglycaemia.