Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease. (14th January 2017)

• Record Type: Journal Article
• Authors: Butterfield, Tiffany R.; Hanna, David B.; Kaplan, Robert C.; Kizer, Jorge R.; Durkin, Helen G.; Young, Mary A.; Nowicki, Marek J.; Tien, Phyllis C.; Golub, Elizabeth T.; Floris-Moore, Michelle A.; Titanji, Kehmia; Fischl, Margaret A.; Heath, Sonya L.; Martinson, Jefferey; Crowe, Suzanne M.; Palmer, Clovis S.; Landay, Alan L.; Anzinger, Joshua J.
• Is Part Of: AIDS. Volume 31:Number 2(2017)
• Publication Date: 2017-01-14
• Subjects: cardiovascular disease -- GLUT1 -- HIV -- monocyte -- T cell; AIDS (Disease) -- Periodicals; Acquired Immunodeficiency Syndrome; AIDS (Disease); Periodicals; Periodicals; 616.9792005
• DOI: 10.1097/QAD.0000000000001320
• Languages: English
• ISSNs: 0269-9370

Abstract

Abstract : Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIV-infected women with subclinical CVD. Methods: Participants with more than 75th percentile ( n = 15) and less than 25th percentile ( n = 15) age-adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 + cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P = 0.024 ) (66.4% vs. 48.5%, P = 0.031 ) and CD38 (339 MFI vs. 211 MFI, P = 0.002 ) (10.5% vs. 3.8%, P = 0.0002 ) compared with women with low IMT. High and low IMT participants showed no differences in GLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 + and CD8 + T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation. Abstract : Supplemental Digital Content is available in the text