Automated Gram-staining characterisation of bacterial cells using colour and cell wall properties. (24th October 2011)

• Record Type: Journal Article
• Authors: Hiremath, P.S.; Bannigidad, Parashuram
• Is Part Of: International journal of biomedical engineering and technology. Volume 7:Number 3(2011)
• Page Start: 257
• Page End: 265
• Publication Date: 2011-10-24
• Subjects: Gram-staining -- colour image analysis -- thresholding -- histogram entropy -- edge detection -- cell wall; Biomedical engineering -- Periodicals; 610.28
• Languages: English
• ISSNs: 1752-6418

Abstract

In this paper, the Gram-staining technique is used as a tool for the differentiation of Gram-positive and Gram-negative bacteria, as a first step to determine the identity of a particular bacterial sample. The automated image analysis has been developed for the monitoring of the Gram-staining characteristics of bacteria based on their colour and cell wall structure. The experimental results are compared with manual results obtained by microbiological expert. The results demonstrate the efficiency of the proposed method.