Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir. (November 2015)

• Record Type: Journal Article
• Authors: Huang, Tinglin; Guo, Lin; Zhang, Haihan; Su, Junfeng; Wen, Gang; Zhang, Kai
• Is Part Of: Bioresource technology. Volume 196(2015)
• Page Start: 209
• Page End: 216
• Publication Date: 2015-11
• Subjects: Aerobic denitrifier -- Response surface methodology -- Reservoir -- Nitrogen removal -- Pseudomonas stutzeri; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.07.059
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: We isolated Pseudomonas stutzeri ZF31 from drinking water reservoir. P. stutzeri ZF31 removed 97% nitrate nitrogen under aerobic condition. Nitrogen balance and denitrification genes determine nitrogen removal mechanism. P. stutzeri ZF31 has potential application for remediation of reservoir water. Abstract: An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16 h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ . Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72 °C, and with shaking at 54.15 rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10 °C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.