Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. (January 2016)

• Record Type: Journal Article
• Authors: Janila, Pasupuleti; Pandey, Manish K; Shasidhar, Yaduru; Variath, Murali T; Sriswathi, Manda; Khera, Pawan; Manohar, Surendra S; Nagesh, Patne; Vishwakarma, Manish K; Mishra, Gyan P; Radhakrishnan, T; Manivannan, N; Dobariya, KL; Vasanthi, RP; Varshney, Rajeev K
• Is Part Of: Plant science. Volume 242(2016:Jan.)
• Page Start: 203
• Page End: 213
• Publication Date: 2016-01
• Subjects: MABC marker-assisted backcrossing -- MAS marker-assisted selection -- ILs introgression lines -- CVD cardiovascular diseases -- LDL low density lipoproteins -- HDL high density lipoproteins -- FAD fatty acid desaturase -- CAPS cleaved amplified polymorphic sequences -- AS-PCR allele specific polymerase chain reaction -- ICGV ICRISAT groundnut variety; Peanut -- Oil content -- Oleic acid -- Oil quality -- Marker-assisted backcrossing -- Marker-assisted selection; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.08.013
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Two molecular breeding approaches namely MABC and MAS were used to enhance oil quality in peanut. Developed high oleate introgression lines; 27 with high and 28 with low oil content. All the selected introgression lines had low linoleic and palmitic acid content. No negative association between high oil content and high oleic acid. Oleic acid increased by 0.5–1.1 folds at par with the donor parent, SunOleic 95R. Abstract: High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 ( ahFAD2A ) and b09 ( ahFAD2B ) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5–1.1 folds, with concomitant reduction of linoleic acid by 0.4–1.0 folds and palmitic acid by 0.1–0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53–58%), and 28 ILs with low oil content (42–50%) were selected that may be released for cultivation upon further evaluation.