Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices. (2016)

• Record Type: Journal Article
• Authors: Poghossian, A.; Bronder, T.S.; Scheja, S.; Wu, C.; Weinand, T.; Metzger-Boddien, C.; Keusgen, M.; Schöning, M.J.
• Is Part Of: Procedia engineering. Volume 168(2016)
• Page Start: 514
• Page End: 517
• Publication Date: 2016
• Subjects: field-effect sensor -- DNA hybridization -- polymerase chain reaction (PCR) -- label-free detection -- polyelectrolyte; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2016.11.512
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.