Supplemental dietary phytosterin protects against 4-nitrophenol-induced oxidative stress and apoptosis in rat testes. (2015)

• Record Type: Journal Article
• Authors: Zhang, Yonghui; Song, Meiyan; Rui, Xiaoli; Pu, Shaoxia; Li, Yansen; Li, ChunMei
• Is Part Of: Toxicology reports. Volume 2(2015)
• Page Start: 664
• Page End: 676
• Publication Date: 2015
• Subjects: EED environmental endocrine disruptor -- PNP 4-nitrophenol -- DEP diesel exhaust particles -- ROS reactive oxygen species -- GSH glutathione -- GSH-Px glutathione peroxidase -- SOD superoxide dismutase -- CAT catalase -- ARE antioxidant response element -- Nrf2 nuclear factor erythroid 2-related factor 2 -- Keap1 kelch-like ECH-associated protein 1 -- PS phytosterins -- HO-1 heme oxygenase 1 -- GCLC γ-glutamylcysteine synthetase -- NQO1 NAD(P)H: quinone oxidoreductase 1 -- NF-κB nuclear factor-κB; 4-Nitrophenol (PubChem CID: 980) -- β-Sitosterol (PubChem CID: 222284) -- Campesterol (PubChem CID: 457801) -- Stigmasterol (PubChem CID: 5280794); 4-Nitrophenol -- Phytosterin -- Nrf2 -- Testis -- Antioxidant gene -- Apoptosis; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2015.04.007
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: 4-Nitrophenol (PNP), is generally regarded as an environmental endocrine disruptor (EED). Phytosterin (PS), a new feed additive, possesses highly efficient antioxidant activities. The transcription factor, nuclear factor-erythroid 2-related factor 2 (Nrf2), is an important regulator of cellular oxidative stress. Using rats, this study examined PNP-induced testicular oxidative damage and PS-mediated protection against that damage. The generation of MDA and H2 O2 upon PNP and PS treatment was milder than that upon treatment with PNP alone. This mitigation was accompanied by partially reversed changes in SOD, CAT, GSH and GSH-Px. Moreover, PNP significantly reduced the caudal epididymal sperm counts and serum testosterone levels. Typical morphological changes were also observed in the testes of PNP-treated animals. PNP reduced the transcriptional level of Nrf2, as evaluated by RT-PCR, but it promoted the dissociation from the Nrf2 complex, stabilization and translocation into the nucleus, as evaluated by immunohistochemistry and Western blotting. In addition, PNP enhanced the Nrf2-dependent gene expression of heme oxygenase-1 (HO-1) and glutamate–cysteine ligase catalytic subunit (GCLC). These results suggest that the Nrf2 pathway plays an important role in PNP-induced oxidative damage and that PS possesses modulatory effects on PNP-induced oxidative damage in rat testes.